Purification Active Antibodies

Precipitation for Non-chromatographic Purification.

After purification, native binding activity is restored with the purified product containing only bivalently active antibodies.

 

Company Name

University of Notre Dame

Reference Number 1249

Market Sectors:

Bio / Life Sciences, Medical

Operational Areas

Patient Healthcare
 

Commercial Solutions

Increase Efficiency
Reduce Cost
 
 

Description:

An affinity-based precipitation method for the rapid and nonchromatographic purification of bivalently active monoclonal antibodies combines the selectivity of affinity chromatography with the simplicity of salt-induced precipitation.  The process demonstrates the ability to purify antibodies from typical contaminants including CHO cell conditioned media, ascites fluid, DNA, and other antibodies with yields >85% and with >95% purity.  Purified antibodies display native binding levels to cell lines expressing the target proteins. Selectivity of the affinity-based precipitation method for bivalent activity can be established by purifying solutions containing both active and chemically denatured antibodies. Prior to purification, solutions can displayed 20−76% reduction in binding activity, where after purification, native binding activity is restored with the purified product containing only bivalently active antibodies.

 Key Features and Benefits:

1.       Reduced purification costs

2.       Increased purified product quality

3.       Affinity-based precipitation method does not adversely affect the antibodies

4.       Rapid and straightforward process for purification of antibodies

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